Anticorps Polyclonal de lapin anti-MTHFD1L

• Phare
• Validé par KD/KO

MTHFD1L Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 16113-1-AP

Synonymes

FTHFSDC1, MTC1THFS, MTHFD1L

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Galerie de données de validation

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  WB analysis using 16113-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 16113-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16113-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16113-1-AP (MTHFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16113-1-AP

  IP result of anti-MTHFD1L (IP:16113-1-AP, 4ug; Detection:16113-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human liver cancer using 16113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16113-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16113-1-AP (MTHFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16113-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16113-1-AP (MTHFD1L antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules COLO 320, cellules HeLa, cellules HepG2, tissu ovarien de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 18 publications below
• IHC: See 2 publications below

Informations sur le produit

16113-1-AP cible MTHFD1L dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTHFD1L Protéine recombinante Ag9036
• Nom complet: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
• Masse moléculaire calculée: 978 aa, 106 kDa
• Poids moléculaire observé: 106 kDa
• Numéro d’acquisition GenBank: BC017477
• Symbole du gène: MTHFD1L
• Identification du gène (NCBI): 25902
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.; Authors - Saara Forsström
human	WB	Cell Metab; Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability.; Authors - Won Dong Lee
human	WB	Nat Metab; Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells; Authors - Alanna C Green
human, mouse	WB	Cell Metab; Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.; Authors - Joni Nikkanen
human	WB, IHC	J Pineal Res; Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.; Authors - Li Cui ; KD Validated
human	WB	Nat Commun; Increased formate overflow is a hallmark of oxidative cancer.; Authors - Johannes Meiser

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Furkan Emre (Verified Customer) (02-21-2022)

I am satisfied with the antibody. It worked as I was expecting from it. I would buy it again. I also add a figure showing chemiluminescence detection of MTHFD1L using your antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human ES2 cells

MTHFD1L Antibody Western Blot validation (1:1000 dilution) in Human ES2 cells (Cat no:16113-1-AP)