Anticorps Polyclonal de lapin anti-MTA3

• Phare
• Validé par KD/KO

MTA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(17)

N° de cat : 14682-1-AP

Synonymes

Metastasis-associated protein MTA3, MTA 3, mta1

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Galerie de données de validation

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  WB analysis using 14682-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 14682-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14682-1-AP

  IP result of anti-MTA3 (IP:14682-1-AP, 4ug; Detection:14682-1-AP 1:3000) with Raji cells lysate 2840 ug.

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  IHC staining of mouse brain using 14682-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14682-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 14682-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U-251 using 14682-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, cellules MCF-7, cellules Raji, tissu de thymus de souris
• Résultats positifs en IP: cellules Raji,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 10 publications below
• IP: See 1 publications below

Informations sur le produit

14682-1-AP cible MTA3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTA3 Protéine recombinante Ag6400
• Nom complet: metastasis associated 1 family, member 3
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 62 kDa, 68 kDa
• Numéro d’acquisition GenBank: BC053631
• Symbole du gène: MTA3
• Identification du gène (NCBI): 57504
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

Publications

Species	Application	Title
mouse	WB	Cell Stem Cell; The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development.; Authors - Jinzhong Qin
mouse		Mol Cell; The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression.; Authors - Susanne Bornelöv
human	IHC	Oncotarget; Metastasis-associated protein 3 in colorectal cancer determines tumor recurrence and prognosis.; Authors - Yi Huang
human	WB,IHC	PLoS One; Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer.; Authors - Li Haiying H ; KD Validated
human	IHC	J Gastroenterol Hepatol; Overexpression of the metastasis-associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma.; Authors - Chuanxi Wang
human	WB,IHC	Tumour Biol; MTA3 regulates malignant progression of colorectal cancer through Wnt signaling pathway.; Authors - Taiwei Jiao

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