Anticorps Polyclonal de lapin anti-MSH3

MSH3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 22393-1-AP

Synonymes

Divergent upstream protein, DUC1, DUG, DUP, hMSH3, Mismatch repair protein 1, MRP1, MSH3, mutS homolog 3 (E. coli)

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Galerie de données de validation

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  WB analysis using 22393-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22393-1-AP (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22393-1-AP

  IP result of anti-MSH3 (IP:22393-1-AP, 4ug; Detection:22393-1-AP 1:1000) with HeLa cells lysate 3600ug.

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  IHC staining of human colon cancer using 22393-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22393-1-AP (MSH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22393-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22393-1-AP (MSH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-29 using 22393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using MSH3 antibody (22393-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

22393-1-AP cible MSH3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MSH3 Protéine recombinante Ag18060
• Nom complet: mutS homolog 3 (E. coli)
• Masse moléculaire calculée: 1137 aa, 127 kDa
• Poids moléculaire observé: 127 kDa
• Numéro d’acquisition GenBank: BC130434
• Symbole du gène: MSH3
• Identification du gène (NCBI): 4437
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

Publications

Species	Application	Title
human	WB	Mol Cancer; CircLIFR synergizes with MSH2 to attenuate chemoresistance via MutSα/ATM-p73 axis in bladder cancer.; Authors - Hui Zhang
human	WB	Sci Adv; Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair; Authors - Dinh Duc Nguyen
human	WB	Hum Mol Genet; Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.; Authors - Dazhang Bai
	WB	bioRxiv; D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability; Authors - Gergely Rona
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona
mouse	WB	Prog Neurobiol; Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice; Authors - Dazhang Bai ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniela (Verified Customer) (03-17-2022)

In western blot we observed multiple bands, but none at the expected molecular weight. In immunofluorescene, staining was citoplasmatic, while MSH3 is expected to be in the nucleus.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for WB; 1:100 for IF
• Cell Tissue Type: Gastric mouse organoids and oesophageal cancer cell line

MSH3 Antibody Western Blot,Immunofluorescence validation (1:1000 for WB; 1:100 for IF dilution) in Gastric mouse organoids and oesophageal cancer cell line (Cat no:22393-1-AP)