Anticorps Polyclonal de lapin anti-MRPS16

• Phare
• Validé par KD/KO

MRPS16 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 16735-1-AP

Synonymes

CGI 132, COXPD2, MRP S16, MRPS16, RPMS16, S16mt

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Galerie de données de validation

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  WB analysis using 16735-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16735-1-AP (MRPS16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16735-1-AP

  WB result of MRPS16 antibody (16735-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS16 transfected HeLa cells.

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  WB analysis of HEK-293 using 16735-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16735-1-AP (MRPS16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16735-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16735-1-AP (MRPS16 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16735-1-AP

  IP result of anti-MRPS16 (IP:16735-1-AP, 4ug; Detection:16735-1-AP 1:500) with HEK-293 cells lysate 1480ug.

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  IF Staining of HepG2 using 16735-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16735-1-AP (MRPS16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 18 publications below

Informations sur le produit

16735-1-AP cible MRPS16 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPS16 Protéine recombinante Ag9749
• Nom complet: mitochondrial ribosomal protein S16
• Masse moléculaire calculée: 137 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC021106
• Symbole du gène: MRPS16
• Identification du gène (NCBI): 51021
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
mouse	WB	Sci Adv; Fidelity of translation initiation is required for coordinated respiratory complex assembly.; Authors - Danielle L Rudler
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
mouse	WB	Nat Commun; Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.; Authors - Tara R Richman
mouse	WB	EMBO J; Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.; Authors - Nicola Ferreira
mouse	WB	Aging Cell; Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.; Authors - Tara R Richman