Anticorps Polyclonal de lapin anti-MRPL23

MRPL23 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11706-1-AP

Synonymes

L23MRP, L23mt, MRP L23, MRPL23, Ribosomal protein L23 like, RPL23, RPL23L

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Galerie de données de validation

• 

  WB analysis of A2780 using 11706-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11706-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11706-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11706-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11706-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11706-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11706-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11706-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11706-1-AP (MRPL23 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 11706-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11706-1-AP (MRPL23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11706-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11706-1-AP (MRPL23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 11706-1-AP

  Immunofluorescent analysis of PC-3 cells using 11706-1-AP (MRPL23 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 11706-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11706-1-AP (MRPL23 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HEK-293, cellules HeLa, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

11706-1-AP cible MRPL23 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPL23 Protéine recombinante Ag2220
• Nom complet: mitochondrial ribosomal protein L23
• Masse moléculaire calculée: 153 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC027710
• Symbole du gène: MRPL23
• Identification du gène (NCBI): 6150
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.; Authors - Tara R Richman
human	WB	Nucleic Acids Res; METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.; Authors - Lindsey Van Haute
mouse	WB	Aging Cell; Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.; Authors - Tara R Richman
mouse	WB	J Cell Biol; Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness.; Authors - Uwe Richter
mouse	WB	PLoS Genet; Mutation in MRPS34 Compromises Protein Synthesis and Causes Mitochondrial Dysfunction.; Authors - Tara R Richman
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi