Anticorps Polyclonal de lapin anti-MRPL13

MRPL13 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16241-1-AP

Synonymes

L13, L13A, L13mt, MRP L13, MRPL13, RPL13, RPML13

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Galerie de données de validation

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  WB analysis using 16241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 16241-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 16241-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16241-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16241-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 16241-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16241-1-AP (MRPL13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 16241-1-AP

  IP Result of anti-MRPL13 (IP:16241-1-AP, 4ug; Detection:16241-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16241-1-AP (MRPL13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules COS-7, cellules HeLa, cellules Jurkat, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

16241-1-AP cible MRPL13 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPL13 Protéine recombinante Ag9105
• Nom complet: mitochondrial ribosomal protein L13
• Masse moléculaire calculée: 178 aa, 21 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC009190
• Symbole du gène: MRPL13
• Identification du gène (NCBI): 28998
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MRPL13, also named as 39S ribosomal protein L13, mitochondrial or L13mt, is a 178 amino acid protein, which belongs to the ribosomal protein L13P family. MRPL13 localizes in the mitochondrion and interacts with OXA1L. MRPL13 is involved in mitochondrial proteins biosynthesis, ribosomal structure and biogenesis.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.; Authors - Elena Lavdovskaia
human	WB	Nucleic Acids Res; Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.; Authors - Surovtseva Yulia V YV
human	WB	Nucleic Acids Res; MPV17L2 is required for ribosome assembly in mitochondria.; Authors - Ilaria Dalla Rosa
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB	J Cell Sci; CLUH couples mitochondrial distribution to the energetic and metabolic status.; Authors - Jamal Wakim
human	WB	Life (Basel); Molecular Investigation of Mitochondrial RNA19 Role in the Pathogenesis of MELAS Disease; Authors - Paola Loguercio Polosa