Anticorps Polyclonal de lapin anti-MPZ / P0

MPZ / P0 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 10572-1-AP

Synonymes

CHM, CMT1, CMT1B, CMT2I, CMT2J, CMT4E, CMTDI3, DSS, HMSNIB, MPP, MPZ, MPZ / P0, Myelin peripheral protein, Myelin protein P0, myelin protein zero, P0

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Galerie de données de validation

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  WB analysis using 10572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10572-1-AP (MPZ / P0 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

10572-1-AP cible MPZ / P0 dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Grenouille
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPZ / P0 Protéine recombinante Ag0848
• Nom complet: myelin protein zero
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC006491
• Symbole du gène: MPZ
• Identification du gène (NCBI): 4359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MPZ (myelin protein zero), also known as P0, is a transmembrane glycoprotein that belongs to the immunoglobulin supergene family. Synthesized by myelin-forming Schwann cells, MPZ is the major structural protein component of myelin in the peripheral nervous system. It is involved in the formation and maintenance of compact myelin, and plays a role in the creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. More than 120 mutations detected in the gene of MPZ cause various forms of hereditary neuropathy, which include Charcot-Marie-Tooth disease type 1B (CMT1B), CMT2, Dejerine-Sottas syndrome (DSS), and congenital hypomyelination neuropathy (CHN). This antibody can recognize endogenous MPZ and can be used as a marker of myelinating Schwann cells. MPZ can be detected the 25-30 kDa band by western blot. A band of 36 kDa could also be detected, which is a novel isoform of MPZ that contains an additional domain at the C-terminal (PMID: 22457349). It can also exist as a dimer (PMID: 12933931).

Publications

Species	Application	Title
rat	WB	Oxid Med Cell Longev; Neuroprotective Effect of Salvianolic Acid A against Diabetic Peripheral Neuropathy through Modulation of Nrf2.; Authors - Chunyang Xu
rat	WB	Front Cell Neurosci; Protein kinase C epsilon activation regulates proliferation, migration, and epithelial to mesenchymal-like transition in rat Schwann cells; Authors - Tasnim Mohamed
human	WB	Front Endocrinol (Lausanne); Clinical identification of expressed proteins in adrenal medullary hyperplasia detected with hypertension; Authors - He Ma
rat	IF	Stem Cell Res Ther; Schwann cells promote prevascularization and osteogenesis of tissue-engineered bone via bone marrow mesenchymal stem cell-derived endothelial cells.; Authors - Xinxin Zhang
rat	WB	Glia; MMP-9 controls Schwann cell proliferation and phenotypic remodeling via IGF-1 and ErbB receptor-mediated activation of MEK/ERK pathway.; Authors - Chattopadhyay Sharmila S
mouse	WB,IF	Mol Cell Biol; DHTKD1 deficiency causes Charcot-Marie-Tooth disease in mice.; Authors - Wang-Yang Xu