Anticorps Polyclonal de lapin anti-MPO

MPO Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

113

N° de cat : 22225-1-AP

Synonymes

myeloperoxidase, EC:1.11.2.2

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Galerie de données de validation

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  WB analysis of HL-60 using 22225-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22225-1-AP (MPO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 22225-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 22225-1-AP (MPO antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 22225-1-AP

  IP result of anti-MPO (IP:22225-1-AP, 4ug; Detection:22225-1-AP 1:4000) with HL-60 cells lysate 1720 ug.

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  IHC staining of human colon cancer using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22225-1-AP (MPO antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22225-1-AP (MPO antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 22225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22225-1-AP (MPO antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human tonsillitis using 22225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 22225-1-AP (MPO antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 22225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 22225-1-AP (MPO antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules U-937
• Résultats positifs en IP: cellules HL-60,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de côlon de souris, tissu hépatique humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 25 publications below
• IHC: See 59 publications below
• IF: See 38 publications below
• ELISA: See 4 publications below
• FC: See 3 publications below

Informations sur le produit

22225-1-AP cible MPO dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPO Protéine recombinante Ag17564
• Nom complet: myeloperoxidase
• Masse moléculaire calculée: 745 aa, 84 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC130476
• Symbole du gène: MPO
• Identification du gène (NCBI): 4353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing. This antibody is specific to MPO.

Publications

Species	Application	Title
	WB	Crit Care; Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.; Authors - Lingbing Zhang
human	IHC	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
	IF	Nat Commun; Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma; Authors - Weiwei Dai
	WB,IHC	Acta Pharm Sin B; A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.; Authors - Ziqiao Yuan
	IHC,IF	Acta Pharm Sin B; A non-human primate derived anti-P-selectin glycoprotein ligand-1 antibody curtails acute pancreatitis by alleviating the inflammatory responses; Authors - Yuhan Li
	IF	Sci Adv; Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage; Authors - Dayun Feng