Anticorps Polyclonal de lapin anti-MON1A

MON1A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 23772-1-AP

Synonymes

MON1 homolog A (yeast), MON1A, SAND1

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Galerie de données de validation

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  WB analysis using 23772-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23772-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 23772-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23772-1-AP (MON1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules K-562, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

23772-1-AP cible MON1A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MON1A Protéine recombinante Ag6900
• Nom complet: MON1 homolog A (yeast)
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 62 kDa and 45 kDa
• Numéro d’acquisition GenBank: BC047022
• Symbole du gène: MON1A
• Identification du gène (NCBI): 84315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MON1A is a cytosolic protein that peripherally associates with the ER. MON1A plays an important role in membrane trafficking through the secretory apparatus. MON1A affects different steps in the secretory pathway including endoplasmic reticulum-to-Golgi traffic. MON1A encodes several isoforms with MW aboout 73, 62, 55, 45 and 34 kDa.

Publications

Species	Application	Title
	WB	Cell Death Dis; TRIM39 deficiency inhibits tumor progression and autophagic flux in colorectal cancer via suppressing the activity of Rab7.; Authors - Jia Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Q (Verified Customer) (06-01-2021)

It can produce a stronger band a little higher than 55. But its identity is not quite sure as in human cells Mon1A is supposed to be around 73 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293 cell lysate

MON1A Antibody Western Blot validation (1:1000 dilution) in HEK293 cell lysate (Cat no:23772-1-AP)