Anticorps Polyclonal de lapin anti-MNAT1

• Phare
• Validé par KD/KO

MNAT1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11719-1-AP

Synonymes

CAP35, CDK7/cyclin H assembly factor, Cyclin G1 interacting protein, MAT1, Menage a trois, MNAT1, p35, p36, RING finger protein 66, RING finger protein MAT1, RNF66, TFB3

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Galerie de données de validation

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  WB analysis using 11719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11719-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11719-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11719-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11719-1-AP (MNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11719-1-AP

  IP result of anti-MNAT1 (IP:11719-1-AP, 4ug; Detection:11719-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of A375 using 11719-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using MNAT1 antibody (11719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HeLa using 11719-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MNAT1 antibody (11719-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A431, cellules HeLa, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules A375, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

11719-1-AP cible MNAT1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MNAT1 Protéine recombinante Ag2293
• Nom complet: menage a trois homolog 1, cyclin H assembly factor (Xenopus laevis)
• Masse moléculaire calculée: 309 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000820
• Symbole du gène: MNAT1
• Identification du gène (NCBI): 4331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Int J Biol Sci; Therapeutic Targeting of CDK7 Suppresses Tumor Progression in Intrahepatic Cholangiocarcinoma.; Authors - Hua-Dong Chen
human	WB,IHC	BMC Cancer; MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway.; Authors - Chensheng Qiu ; KD Validated
mouse	WB	Acta Histochem; Cyclin-dependent kinase 5 regulates MAPK/ERK signaling in the skin of mice.; Authors - Xuexian Liu
mouse	WB	Genes Dev; TRF1 uses a noncanonical function of TFIIH to promote telomere replication; Authors - Zhe Yang