Anticorps Polyclonal de lapin anti-MMP28

• Phare
• Validé par KD/KO

MMP28 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 18237-1-AP

Synonymes

EPILYSIN, matrix metallopeptidase 28, Matrix metalloproteinase 28, MM28, MMP 28, MMP28

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Galerie de données de validation

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  WB analysis of A549 using 18237-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18237-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 18237-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 18237-1-AP (MMP28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18237-1-AP (MMP28 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 18237-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 18237-1-AP (MMP28 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 18237-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 18237-1-AP (MMP28 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, tissu cardiaque de souris, tissu de côlon humain
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de la peau humain, tissu ovarien humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

18237-1-AP cible MMP28 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP28 Protéine recombinante Ag12980
• Nom complet: matrix metallopeptidase 28
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC002631
• Symbole du gène: MMP28
• Identification du gène (NCBI): 79148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP28(Epilysin)can degrade casein and play a role in tissues homeostasis and repair .The only specific human cell type recognized to express epilysin is the basal keratinocyte in the skin, where epilysin expression is upregulated during wound repair(PMID:16940349).Western blot showed this antibody recognize bands at 62 and 58 kDa as well as breakdown products at 50, 48, and 46 kDa(PMID:15953044).This antibody is specific to MMP28.

Publications

Species	Application	Title
mouse	IHC,IF	Cell Mol Life Sci; Microglial activation in an amyotrophic lateral sclerosis-like model caused by Ranbp2 loss and nucleocytoplasmic transport impairment in retinal ganglion neurons.; Authors - Kyoung-In Cho
human	WB	FASEB J; Drastic induction of MMP-7 by cortisol in the human amnion: implications for membrane rupture at parturition.; Authors - Lu-Yao Wang
human	WB	Am J Pathol; CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2.; Authors - Zhang Min M
mouse	WB,IF	Dis Model Mech; Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.; Authors - Kyoung-In Cho
human	IHC	Am J Pathol; Matrix Metalloproteinase-28 Is a Key Contributor to Emphysema Pathogenesis.; Authors - Anne M Manicone
mouse	IF	JBMR Plus; Preexisting Type 1 Diabetes Mellitus Blunts the Development of Posttraumatic Osteoarthritis.; Authors - Naiomy D Rios-Arce