Anticorps Polyclonal de lapin anti-MMP19

MMP19 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14244-1-AP

Synonymes

matrix metallopeptidase 19, Matrix metalloproteinase 18, Matrix metalloproteinase 19, Matrix metalloproteinase RASI, MMP 18, MMP 19, MMP18, MMP19, RASI, RASI 1

---

Galerie de données de validation

• 

  WB analysis of SH-SY5Y using 14244-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14244-1-AP (MMP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14244-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14244-1-AP (MMP19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 14244-1-AP

  IP result of anti-MMP19 (IP:14244-1-AP, 4ug; Detection:14244-1-AP 1:500) with human placenta tissue lysate 1520ug.

• 

  IHC staining of human liver cancer using 14244-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14244-1-AP (MMP19 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 14244-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14244-1-AP (MMP19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 14244-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using MMP19 antibody (14244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu placentaire humain
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

14244-1-AP cible MMP19 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP19 Protéine recombinante Ag5501
• Nom complet: matrix metallopeptidase 19
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-60 kDa
• Numéro d’acquisition GenBank: BC050368
• Symbole du gène: MMP19
• Identification du gène (NCBI): 4327
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP19(matrix metalloproteinase-19) has the capacity to degrade several basement membrane proteins such as type IV collagen, laminin, tenascin C, and nidogen. It is expressed by microvascular endothelial cells in vitro as well as by capillaries in the inflamed synovial membrane, whereas its expression is absent in quiescent macrovascular endothelial cells of large arteries and veins . And it is also detected in capillaries of normal mammary tissue, benign mammary carcinomas and in skin capillaries(PMID:15241558).

Publications

Species	Application	Title
	WB	Bioact Mater; Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis; Authors - Wei Zhang
mouse	WB	Cancer Gene Ther; A critical ETV4/Twist1/Vimentin axis in Ha-RAS-induced aggressive breast cancer.; Authors - Wuling Liu
human	WB,IHC	J Cell Biochem; MicroRNA-193b-3p Regulates Matrix Metalloproteinase 19 Expression in Interleukin-1β-Induced Human Chondrocytes.; Authors - Zong-Kun Chang
human	WB	Front Pharmacol; METTL3/m6A/IFIT2 regulates proliferation, invasion and immunity in esophageal squamous cell carcinoma; Authors - Fangfang Ge
human	WB	J Biochem Mol Toxicol; Pacenta polypeptide injection alleviates the fibrosis and inflammation in cigarette smoke extracts-induced BEAS-2B cells by modulating MMP-9/TIMP-1 signaling; Authors - Wei Xu
rat	WB	J Immunol Res; Dissecting the Molecular Mechanism of Wang-Bi Capsule in the Treatment of Experimental Rheumatoid Arthritis Based on Synovial Tissue Proteomic Analysis.; Authors - Haiyang Shu