Anticorps Polyclonal de lapin anti-MMP14 / MT1-MMP

MMP14 / MT1-MMP Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 14552-1-AP

Synonymes

Matrix metalloproteinase 14, MMP 14, MMP X1, MMP14, MT MMP 1, MT1 MMP, MT1MMP, MT1-MMP, MTMMP1

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Galerie de données de validation

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  WB analysis of HepG2 using 14552-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14552-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14552-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 14552-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 14552-1-AP (MT1-MMP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 14552-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14552-1-AP (MMP14 / MT1-MMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14552-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14552-1-AP (MMP14 / MT1-MMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14552-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14552-1-AP (MT1-MMP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of COLO 320 using 14552-1-AP

  Immunofluorescent analysis of COLO 320 cells using 14552-1-AP (MT1-MMP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules COLO 320, tissu de côlon humain
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules COLO 320

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Informations sur le produit

14552-1-AP cible MMP14 / MT1-MMP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP14 / MT1-MMP Protéine recombinante Ag5965
• Nom complet: matrix metallopeptidase 14 (membrane-inserted)
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 66 kDa, 45-50 kDa
• Numéro d’acquisition GenBank: BC064803
• Symbole du gène: MMP14
• Identification du gène (NCBI): 4323
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP14, also named as MT1-MMP, is a key matrix metalloproteinase (MMP) family member which plays a crucial role in tumor growth, invasion and metastasis. MT1-MMP is a cell membrane-bound proteinase, and it enhances degradation of collagen IV, a major component of the basement membrane, by forming a complex with tissue inhibitor of metalloproteinase-2 (TIMP-2) to activate pro-MMP-2. MT1-MMP can influence venous invasion, intrahepatic metastasis , and patient outcome in hepatocellular carcinoma (HCC). MT1-MMP was reported to be present in centromere and could lead to chromosome instability in MDCK cells, indicating that MT1-MMP may have more novel functions in the intracellular compartments. In western blotting, pro-MMP14 (65 kDa) and MMP14 (51 kDa) bands showed with the truncated MMP14 (45, 42, 35, 20 kDa) forms (PMID:12097451).

Publications

Species	Application	Title
human,rat	WB,IF	J Neuroinflammation; SPOCK2 modulates neuropathic pain by interacting with MT1-MMP to regulate astrocytic MMP-2 activation in rats with chronic constriction injury; Authors - Chenglong Wang
mouse	WB	Mol Ther; METTL3-mediated m6A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.; Authors - Ling Jiang
human	WB	Int J Mol Sci; PLOD2 Is a Prognostic Marker in Glioblastoma That Modulates the Immune Microenvironment and Tumor Progression.; Authors - Nina Kreße
human	WB	Int J Mol Sci; LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases.; Authors - Manabu Ogoyama
human	WB	Cancers (Basel); WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.; Authors - Nathalie Ferrand
human	WB	Cancer Lett; Long non-coding RNA MALAT1 promotes gastric cancer tumorigenicity and metastasis by regulating vasculogenic mimicry and angiogenesis.; Authors - Yue Li