Anticorps Polyclonal de lapin anti-MMAB

MMAB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11137-1-AP

Synonymes

ATR, cblB, MMAB

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Galerie de données de validation

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  WB analysis of HeLa using 11137-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11137-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11137-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11137-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11137-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11137-1-AP (MMAB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11137-1-AP

  IP result of anti-MMAB (IP:11137-1-AP, 4ug; Detection:11137-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human ovary tumor using 11137-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11137-1-AP (MMAB antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11137-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MMAB antibody (11137-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules BxPC-3, cellules HepG2, cellules L02
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

11137-1-AP cible MMAB dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMAB Protéine recombinante Ag1602
• Nom complet: methylmalonic aciduria (cobalamin deficiency) cblB type
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC011831
• Symbole du gène: MMAB
• Identification du gène (NCBI): 326625
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MMAB gene encodes cob(I)alamin adenosyltransferase, which catalyzes the final step in the synthesis of the coenzyme adenosylcobalamin (AdoCbl). AdoCbl is a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. The deduced 250-amino acid protein has a calculated molecular mass of 27.3 kD.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Pharmacological chaperones as a potential therapeutic option in methylmalonic aciduria cblB type.; Authors - Jorge-Finnigan Ana A
human	WB	Hum Mutat; Functional and structural analysis of five mutations identified in methylmalonic aciduria cblB type.; Authors - Jorge-Finnigan Ana A
human	WB,IF	Biochim Biophys Acta Mol Basis Dis; Hepatocyte-like cells differentiated from methylmalonic aciduria cblB type induced pluripotent stem cells: A platform for the evaluation of pharmacochaperoning.; Authors - Á Briso-Montiano
	WB	Biochim Biophys Acta; New perspectives for pharmacological chaperoning treatment in methylmalonic aciduria cblB type.; Authors - S Brasil