Anticorps Polyclonal de lapin anti-MLLT3/AF9

MLLT3/AF9 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 12825-1-AP

Synonymes

AF9, FLJ2035, MLLT3, Protein AF 9, YEATS3

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Galerie de données de validation

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  WB analysis of Jurkat using 12825-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12825-1-AP (MLLT3/AF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12825-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12825-1-AP (MLLT3/AF9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12825-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12825-1-AP (MLLT3/AF9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12825-1-AP cible MLLT3/AF9 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MLLT3/AF9 Protéine recombinante Ag3528
• Nom complet: myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
• Masse moléculaire calculée: 568 aa, 63 kDa
• Poids moléculaire observé: 63-70 kDa
• Numéro d’acquisition GenBank: BC036089
• Symbole du gène: AF9/MLLT3
• Identification du gène (NCBI): 4300
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MLLT3, also named as AF9, YEATS3, is a 568 amino acid protein, which is a component of the superelongation complex6 and co-operates with DOT1L, which di/trimethylates H3K79 to promote transcription. MLLT3 localizes to active transcription start sites (TSSs) through the YEATS domain, which recognizes active histone marks such as H3K9 acetylation and crotonylation. A truncated MLLT3 that lacks the YEATS domain forms a leukaemic fusion protein with the N terminus of MLL1, which misdirects MLLT3-interacting complexes to induce aberrant gene transcription. MLLT3 also regulates erythroid or megakaryocytic progenitors and was identified as a definitive HSC hub gene during mouse development. Researcher found that the expression of a gene called MLLT3 was closely correlated with blood stem cells'potential to self-renew and that the protein generated by the MLLT3 gene provides blood stem cells with the instructions necessary to maintain its ability to self-renew. It does this by working with other regulatory proteins to keep important parts of the blood stem cell's machinery operational as the cells divide.