Anticorps Polyclonal de lapin anti-MIXL1

MIXL1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 22772-1-AP

Synonymes

hMix, Homeobox protein MIXL1, Homeodomain protein MIX, MILD1, MIX, Mix.1 homeobox like protein, MIX1 homeobox like protein 1, MIXL, MIXL1

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Galerie de données de validation

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  WB analysis of HEK-293 using 22772-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 22772-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of H7ES using 22772-1-AP

  H7ES cells were subjected to SDS PAGE followed by western blot with 22772-1-AP (MIXL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 22772-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 22772-1-AP (MIXL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu pancréatique de rat
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 6 publications below

Informations sur le produit

22772-1-AP cible MIXL1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIXL1 Protéine recombinante Ag18732
• Nom complet: Mix1 homeobox-like 1 (Xenopus laevis)
• Masse moléculaire calculée: 232 aa, 25 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC113441
• Symbole du gène: MIXL1
• Identification du gène (NCBI): 83881
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mix1 homeobox-like 1 (MIXL1) Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression. This antibody is specific to MIXL1.

Publications

Species	Application	Title
human	IF	Cell Stem Cell; Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.; Authors - Jian Hui Low
human	IF	Adv Healthc Mater; Spatial Stem Cell Fate Engineering via Facile Morphogen Localization; Authors - Gyuhyung Jin
		Cell Death Differ; Uric acid: a potent molecular contributor to pluripotent stem cell cardiac differentiation via mesoderm specification.; Authors - Bingbing Ke
human	IF	Int J Mol Sci; Porcine Kidney Organoids Derived from Naïve-like Embryonic Stem Cells; Authors - Meishuang Li
pig	IF	Front Cell Dev Biol; Species origin of exogenous transcription factors affects the activation of endogenous pluripotency markers and signaling pathways of porcine induced pluripotent stem cells; Authors - Meng Zhou
human	IF	STAR Protoc; Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells.; Authors - Julie Bejoy