Anticorps Polyclonal de lapin anti-MINA

• Phare
• Validé par KD/KO

MINA Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12214-1-AP

Synonymes

MDIG, MINA, Mina53, MYC induced nuclear antigen, NO52, Nucleolar protein 52, Ribosomal oxygenase 2, RIOX2, ROX

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Galerie de données de validation

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  WB analysis of HEK-293 using 12214-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12214-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12214-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12214-1-AP (MINA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12214-1-AP

  IP result of anti-MINA (IP:12214-1-AP, 3ug; Detection:12214-1-AP 1:500) with HEK-293 cells lysate 2120ug.

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  IHC staining of human pancreas cancer using 12214-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12214-1-AP (MINA antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A375, cellules A431, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12214-1-AP cible MINA dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MINA Protéine recombinante Ag2838
• Nom complet: MYC induced nuclear antigen
• Masse moléculaire calculée: 465 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC014928
• Symbole du gène: MINA
• Identification du gène (NCBI): 84864
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MINA, also named as MDIG, MINA53 and NO52, belongs to the MINA53/NO66 family. It is involved in cellular proliferation. MINA may play an important role in cell growth and survival. It is involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. The transcriptional repressor MINA has been shown to repress Il4 gene expression by directly binding to its promoter, thereby, controlling TH2 bias.(PMID:21499227)

Publications

Species	Application	Title
mouse	WB,IF	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du ; KD Validated
mouse	WB	EMBO J; NFIL3/E4BP4 controls type 2 T helper cell cytokine expression.; Authors - Kashiwada Masaki M
human	WB	Cancer Res; ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.; Authors - Lili Zhang
human	WB	Oncol Lett; Effects of mdig on proliferation and apoptosis of lung cancer cells.; Authors - Xiaohui Xu ; KD Validated