Anticorps Polyclonal de lapin anti-MIER3

MIER3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17543-1-AP

Synonymes

Mi er3, MIER3

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Galerie de données de validation

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  WB analysis of mouse uterus using 17543-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17543-1-AP (MIER3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 17543-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 17543-1-AP (MIER3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu utérin de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

17543-1-AP cible MIER3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MIER3 Protéine recombinante Ag11604
• Nom complet: mesoderm induction early response 1, family member 3
• Masse moléculaire calculée: 522 aa, 59 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC041348
• Symbole du gène: MIER3
• Identification du gène (NCBI): 166968
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mesoderm induction early response (MIER) protein family is known as fibroblast growth factor (FGF)-regulated immediate-early protein family. Activated by FGF, MIER proteins may have a pivotal role in FGF-regulated cellular activities, indicating a potential influence in the progression of certain cancers [PMID:15117948]. MIER3 localizes to the nucleus and primarily functions as a transcriptional repressor. Rat mammary carcinoma susceptibility 1b (Mcs1b) is a quantitative trait locus on RN02 that confers decreased susceptibility when Copenhagen (COP)-resistant alleles are introgressed into a Wistar Furth (WF)-susceptible genome. MIER3 is a candidate Mcs1b gene [PMID:22993404].

Publications

Species	Application	Title
mouse	WB	Mitochondrion; Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5.; Authors - Shih-Wei Chen