Anticorps Polyclonal de lapin anti-MICAL2

• Phare
• Validé par KD/KO

MICAL2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 13965-1-AP

Synonymes

KIAA0750, MICAL2, MICAL2PV1, MICAL2PV2, Protein MICAL 2

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Galerie de données de validation

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  WB analysis using 13965-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13965-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13965-1-AP (MICAL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 13965-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13965-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13965-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13965-1-AP (MICAL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13965-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 13965-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MICAL2 antibody (13965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules MG U-87, cellules PC-3, cellules U-251
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13965-1-AP cible MICAL2 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MICAL2 Protéine recombinante Ag4950
• Nom complet: microtubule associated monoxygenase, calponin and LIM domain containing 2
• Masse moléculaire calculée: 127 kDa
• Poids moléculaire observé: 95 kDa, 112 kDa
• Numéro d’acquisition GenBank: BC044577
• Symbole du gène: MICAL2
• Identification du gène (NCBI): 9645
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions. There are three known isoforms, MICAL1, MICAL2, and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain, and a Lin11, Isl-1, and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variant of MICAL2, has been reported to be involved in cancer progression of prostate cancer. This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.

Publications

Species	Application	Title
human	WB	Theranostics; Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction.; Authors - Chunmei Xu
human	WB,CoIP,IHC,IF	Oncogene; Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement; Authors - Ze Zhang ; KD Validated
		Cancer Lett; MICAL2 is a novel nucleocytoplasmic shuttling protein promoting cancer invasion and growth of lung adenocarcinoma.; Authors - Wolong Zhou
human	WB	Oxid Med Cell Longev; MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation; Authors - Chenxiang Qi ; KD Validated
human	WB	Acta Physiol (Oxf); MICAL2 Promotes Breast Cancer Cell Migration by Maintaining EGFR Stability and EGFR/P38 Signaling Activation.; Authors - Yueyuan Wang ; KD Validated
human	WB	Front Mol Biosci; MICAL2 Facilitates Gastric Cancer Cell Migration via MRTF-A-Mediated CDC42 Activation.; Authors - Yueyuan Wang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kincaid (Verified Customer) (06-02-2024)

H4 cell lysate run on SDS-PAGE gel. aMICAL2 diluted in 3% BSA, in TBS-tween (0.1%).

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: H4 cells

MICAL2 Antibody Western Blot validation (1:500 dilution) in H4 cells (Cat no:13965-1-AP)