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Anticorps Polyclonal de lapin anti-MICA

MICA Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 12619-1-AP

Synonymes

MIC A, MICA, PERB11.1

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using MICA Polyclonal antibody (12619-1-AP)

    WB analysis using 12619-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12619-1-AP (MICA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MICA Polyclonal antibody (12619-1-AP)

    WB analysis of HeLa using 12619-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12619-1-AP (MICA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules K-562, cellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

WBSee 2 publications below

Informations sur le produit

12619-1-AP cible MICA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéeHumain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène MICA Protéine recombinante Ag3298
Nom complet MHC class I polypeptide-related sequence A
Masse moléculaire calculée 383 aa, 43 kDa
Poids moléculaire observé 43 kDa, 50-55 kDa
Numéro d’acquisition GenBankBC016929
Symbole du gène MICA
Identification du gène (NCBI) 100507436
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531).

Protocole

Product Specific Protocols
WB protocol for MICA antibody 12619-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Inhibition of Checkpoint Kinase 1 (CHK1) Upregulates Interferon Regulatory Factor 1 (IRF1) to Promote Apoptosis and Activate Anti-Tumor Immunity via MICA in Hepatocellular Carcinoma (HCC)

Authors - Xicai Li
View Article
humanWB

Cancers (Basel)

MICA+ Tumor Cell Upregulated Macrophage-Secreted MMP9 via PROS1-AXL Axis to Induce Tumor Immune Escape in Advanced Hepatocellular Carcinoma (HCC)

Authors - Qiulin Wu
View Article