Anticorps Polyclonal de lapin anti-MGMT

• Phare
• Validé par KD/KO

MGMT Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), IP, CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

20

N° de cat : 17195-1-AP

Synonymes

O-6-methylguanine-DNA-alkyltransferase, Methylated-DNA--protein-cysteine methyltransferase, EC:2.1.1.63, 6-O-methylguanine-DNA methyltransferase

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Galerie de données de validation

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  WB analysis using 17195-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17195-1-AP (MGMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17195-1-AP

  WB result of MGMT antibody (17195-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGMT transfected HeLa cells.

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  WB analysis of Jurkat using 17195-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17195-1-AP (MGMT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17195-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17195-1-AP (MGMT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17195-1-AP

  IP result of anti-MGMT (IP:17195-1-AP, 4ug; Detection:17195-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of human liver cancer using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17195-1-AP (MGMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17195-1-AP (MGMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17195-1-AP (MGMT antibody) at dilution of 1:100 (under 40x lens).

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  FC experiment of Jurkat using 17195-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human MGMT (17195-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules MCF-7, cellules Raji
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu placentaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17195-1-AP cible MGMT dans les applications de WB, IHC, FC (Intra), IP, CoIP, ELISA, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MGMT Protéine recombinante Ag9996
• Nom complet: O-6-methylguanine-DNA methyltransferase
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC000824
• Symbole du gène: MGMT
• Identification du gène (NCBI): 4255
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MGMT is the primary vehicle for cellular removal of alkyl lesions from the O-6 position of guanine and the O-4 position of thymine. While key to the maintenance of genomic integrity, MGMT also removes damage induced by alkylating chemotherapies, inhibiting the efficacy of cancer treatment [PMID:23065697].MGMT is the primary mechanism for the removal of alkylation damage from the O-6 position of guanine [PMID: 17482892]. The O-6 position of guanine is one of several positions in DNA bases to which alkyl groups are attached in SN1 alkylation reactions, and this repair has been well-characterized in mammalian cells and via MGMT homologs in bacteria and Archaea.[PMID: 10767620]

Publications

Species	Application	Title
human	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB	Oncogene; GBP3 promotes glioblastoma resistance to temozolomide by enhancing DNA damage repair.; Authors - Hui Xu
human	IHC	Oncogenesis; GSK3β palmitoylation mediated by ZDHHC4 promotes tumorigenicity of glioblastoma stem cells in temozolomide-resistant glioblastoma through the EZH2-STAT3 axis.; Authors - Chenggang Zhao
human	WB	Arch Toxicol; Global and MGMT promoter hypomethylation independently associated with genomic instability of lymphocytes in subjects exposed to high-dose polycyclic aromatic hydrocarbon.; Authors - Duan Huawei H