Anticorps Polyclonal de lapin anti-MFI2

MFI2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 10428-1-AP

Synonymes

Melanotransferrin, MFI2, Mtf, p97

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Galerie de données de validation

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  WB analysis of HEK-293 using 10428-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10428-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 10428-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, transfectées

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

10428-1-AP cible MFI2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MFI2 Protéine recombinante Ag0700
• Nom complet: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 82 kDa
• Numéro d’acquisition GenBank: BC001875
• Symbole du gène: MFI2
• Identification du gène (NCBI): 4241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MFI2 (Melanotransferrin/Mtf/TRMF), also known as p97 or CD228, is an iron-binding glycoprotein belonging to the transferrin family. Alternative RNA splicing yields two forms of different length transcripts, one (the longer) bound to the membrane (mMFI2) and the other (the shorter) secreted to the peripheral environment (sMFI2) such as blood and cell supernatant. MFI2 is upregulated in tumor tissues and has been identified as a serological Marker of colorectal cancer (PMID: 25216327).

Publications

Species	Application	Title
mouse	WB	Cell Death Discov; Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling; Authors - Ping Yin
human,rat	WB,IF	Reprod Sci; MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration; Authors - Yanhong Mao
human	WB	Front Immunol; Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis; Authors - Jianfeng Shu