Anticorps Polyclonal de lapin anti-METTL16

• Phare
• Validé par KD/KO

METTL16 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 19924-1-AP

Synonymes

METT10D, METTL16

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Galerie de données de validation

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  WB analysis using 19924-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19924-1-AP (METTL16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 19924-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 19924-1-AP (METTL16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 19924-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19924-1-AP (METT10D antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 19924-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19924-1-AP (METTL16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 19924-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19924-1-AP (METTL16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 19924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19924-1-AP (METTL16 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NCI-H1299, cellules NIH/3T3, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 4 publications below

Informations sur le produit

19924-1-AP cible METTL16 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: METTL16 Protéine recombinante Ag13889
• Nom complet: methyltransferase 10 domain containing
• Masse moléculaire calculée: 562 aa, 64 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC001213
• Symbole du gène: METTL16
• Identification du gène (NCBI): 79066
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methyltransferase-like protein 16 (METTL16) is a human RNA methyltransferase that installs m6A marks on U6 small nuclear RNA (U6 snRNA) and S-adenosylmethionine (SAM) synthetase pre-mRNA. METTL16 is considered a protective gene, suppressing the development of OC, HCC, and endocrine system tumors (PMID: 33237039, PMID: 33133142, PMID: 34187307). However, the high expression of METTL16 has been linked with a poor survival rate in patients with breast cancer (PMID: 33362863). METTL16 has 2 isoforms with molecular weights of 64 and 26 kDa.

Publications

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	Oxid Med Cell Longev; Methylation Pattern Mediated by m6A Regulator and Tumor Microenvironment Invasion in Lung Adenocarcinoma.; Authors - Feng Jiang
human	WB	J Biol Chem; m6A demethylation of cytidine deaminase APOBEC3B mRNA orchestrates arsenic-induced mutagenesis.; Authors - Ming Gao
human	WB,IHC	Anal Cell Pathol (Amst); METTL16 Inhibits the Malignant Progression of Epithelial Ovarian Cancer through the lncRNA MALAT1/β-Catenin Axis; Authors - Changshu Li ; KD Validated
human	IHC	J Coll Physicians Surg Pak; Clinical Significance of Methyltransferase-like 16 Expression in Epithelial Ovarian Cancer; Authors - Changshu Li