Anticorps Polyclonal de lapin anti-MEPE

• Phare
• Validé par KD/KO

MEPE Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 18804-1-AP

Synonymes

MEPE, OF45, Osteoblast/osteocyte factor 45

---

Galerie de données de validation

• 

  WB analysis of HeLa using 18804-1-AP

  WB result of MEPE antibody (18804-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPE transfected HeLa cells.

• 

  WB analysis of HepG2 using 18804-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 18804-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18804-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18804-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 18804-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A2780, cellules A375, cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18804-1-AP cible MEPE dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: matrix extracellular phosphoglycoprotein
• Masse moléculaire calculée: 525 aa, 58 kDa
• Poids moléculaire observé: 43 kDa, 98 kDa
• Numéro d’acquisition GenBank: BC128158
• Symbole du gène: MEPE
• Identification du gène (NCBI): 56955
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).