Anticorps Polyclonal de lapin anti-MELK

MELK Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11403-1-AP

Synonymes

hMELK, HPK38, KIAA0175, MELK, Protein kinase PK38

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Galerie de données de validation

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  WB analysis of Jurkat using 11403-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 11403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11403-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11403-1-AP (MELK Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11403-1-AP (MELK Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11403-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11403-1-AP (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de côlon de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11403-1-AP cible MELK dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MELK Protéine recombinante Ag1897
• Nom complet: maternal embryonic leucine zipper kinase
• Masse moléculaire calculée: 651 aa, 75 kDa
• Poids moléculaire observé: 50-70 kDa
• Numéro d’acquisition GenBank: BC014039
• Symbole du gène: MELK
• Identification du gène (NCBI): 9833
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

Publications

Species	Application	Title
human	WB,IHC,IF,CoIP	Int J Oncol; MELK predicts poor prognosis and promotes metastasis in esophageal squamous cell carcinoma via activating the NF‑κB pathway.; Authors - Jiecheng Ye
human	IHC	Med Sci Monit; Up-Regulated Maternal Embryonic Leucine Zipper Kinase Predicts Poor Prognosis of Hepatocellular Carcinoma Patients in a Chinese Han Population.; Authors - Shaohan Wu
human	IHC	Drug Des Devel Ther; A Novel Tetramethylpyrazine Chalcone Hybrid- HCTMPPK, as a Potential Anti-Lung Cancer Agent by Downregulating MELK; Authors - Yan Ma