Anticorps Polyclonal de lapin anti-MEFV

MEFV Polyclonal Antibody for WB, IP, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 24280-1-AP

Synonymes

Pyrin

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Galerie de données de validation

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  WB analysis of human plasma using 24280-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24280-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 24280-1-AP

  IP result of anti-MEFV (IP:24280-1-AP, 4ug; Detection:24280-1-AP 1:5000) with THP-1 cells lysate 1820 ug.

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  IF Staining of A431 using 24280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MEFV antibody (24280-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of A431 using 24280-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MEFV (24280-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules THP-1, plasma humain
• Résultats positifs en IP: cellules THP-1,
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431
• Résultats positifs en cytométrie: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

24280-1-AP cible MEFV dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEFV Protéine recombinante Ag18000
• Nom complet: Mediterranean fever
• Masse moléculaire calculée: 781 aa, 86 kDa
• Poids moléculaire observé: 86 kDa
• Numéro d’acquisition GenBank: BC101511
• Symbole du gène: MEFV
• Identification du gène (NCBI): 4210
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEFV, a gene encoding a protein of unknown function, is the genetic cause of Familial Mediterranean fever (FMF), a recessively inherited disorder characterized by recurring attacks of fever and serositis. Recently mutation of MEFV has been reported to cause autoinflammatory disease distinct from FMF.

Publications

Species	Application	Title
mouse	WB,IP	Autophagy; Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes; Authors - Nivedita Hegdekar
rat	IF	Exp Ther Med; Function and mechanism of pyrin and IL-10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock.; Authors - Xin Jin
human	FC	PLoS Biol; Transcriptional licensing is required for Pyrin inflammasome activation in human macrophages and bypassed by mutations causing familial Mediterranean fever; Authors - Matthew S J Mangan
		Nature; FcγR-mediated SARS-CoV-2 infection of monocytes activates inflammation; Authors - Caroline Junqueira