Anticorps Polyclonal de lapin anti-MEF2C-Specific

MEF2C-Specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 18293-1-AP

Synonymes

MEF2C, MEF2C-Specific, myocyte enhancer factor 2C

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 18293-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 18293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18293-1-AP (MEF2C-Specific antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y,
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

18293-1-AP cible MEF2C-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myocyte enhancer factor 2C
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 45-70 kDa
• Numéro d’acquisition GenBank: NM_002397
• Symbole du gène: MEF2C
• Identification du gène (NCBI): 4208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. The immunogen used is peptide from the specific sequecne of MEF2C transcript variant 1 match to transcript variant 5. The antibody can recognize the transcript variant 1, transcript variant 2 and transcript variant 3 of MEF2C.