Anticorps Polyclonal de lapin anti-MEF2C

MEF2C Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

N° de cat : 20326-1-AP

Synonymes

MEF2C, myocyte enhancer factor 2C

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Galerie de données de validation

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  WB analysis of HeLa using 20326-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20326-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20326-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 20326-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lymph using 20326-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 20326-1-AP (MEF2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 20326-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 20326-1-AP (MEF2C Antibody) at dilution of 1:50.

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  IHC staining of human heart using 20326-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 20326-1-AP (MEF2C Antibody) at dilution of 1:50.

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  IF Staining of mouse brain using 20326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (20326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 20326-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (20326-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules SH-SY5Y, tissu d'intestin grêle de souris, tissu lymphatique de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

20326-1-AP cible MEF2C dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEF2C Protéine recombinante Ag13255
• Nom complet: myocyte enhancer factor 2C
• Masse moléculaire calculée: 469 aa, 51 kDa
• Poids moléculaire observé: 45-70 kDa
• Numéro d’acquisition GenBank: BC026341
• Symbole du gène: MEF2C
• Identification du gène (NCBI): 4208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.