Anticorps Polyclonal de lapin anti-MEF2C

MEF2C Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, ChIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 18290-1-AP

Synonymes

MEF2C, myocyte enhancer factor 2C

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Galerie de données de validation

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  WB analysis using 18290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18290-1-AP (MEF2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18290-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18290-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 18290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (18290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 18290-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MEF2C antibody (18290-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral humain, tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

18290-1-AP cible MEF2C dans les applications de WB, IHC, IF-P, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myocyte enhancer factor 2C
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 45-70 kDa
• Numéro d’acquisition GenBank: NM_002397
• Symbole du gène: MEF2C
• Identification du gène (NCBI): 4208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.

Publications

Species	Application	Title
		Nature; Genetic and epigenetic coordination of cortical interneuron development.; Authors - Kathryn C Allaway ; KO Validated
human	ChIP	Nature; Evolution of Osteocrin as an activity-regulated factor in the primate brain.; Authors - Bulent Ataman
human	IHC	Biol Reprod; Integrated microRNA and mRNA network analysis of the human myometrial transcriptome in the transition from quiescence to labor.; Authors - William E Ackerman
mouse	IF	Cell Rep; Chromatin Environment and Cellular Context Specify Compensatory Activity of Paralogous MEF2 Transcription Factors.; Authors - Shahriyar P Majidi
human	ChIP	Cell Genom; Single nucleus multiomics identifies ZEB1 and MAFB as candidate regulators of Alzheimer's disease-specific cis-regulatory elements; Authors - Ashlyn G Anderson
chicken	IHC	Int J Mol Sci; miR-1 as a Key Epigenetic Regulator in Early Differentiation of Cardiac Sinoatrial Region; Authors - Carlos García-Padilla