Anticorps Polyclonal de lapin anti-MED28

MED28 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 16262-1-AP

Synonymes

1500003D12Rik, EG1, Endothelial derived protein 1, magicin, MED28, mediator complex subunit 28, Tumor angiogenesis marker EG 1

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Galerie de données de validation

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  WB analysis using 16262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 16262-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules PC-12, tissu testiculaire de rat
• Résultats positifs en IHC: tissu testiculaire de souris, tissu cérébral de souris, tissu cérébral humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16262-1-AP cible MED28 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MED28 Protéine recombinante Ag9327
• Nom complet: mediator complex subunit 28
• Masse moléculaire calculée: 178 aa, 20 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC011936
• Symbole du gène: MED28
• Identification du gène (NCBI): 80306
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.