Anticorps Polyclonal de lapin anti-MDMX

• Phare
• Validé par KD/KO

MDMX Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA, IF

Conjugaison

Non conjugué

Publications

29

Avis (2)

N° de cat : 17914-1-AP

Synonymes

MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

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Galerie de données de validation

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  WB analysis of HeLa using 17914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Apoptosised HeLa using 17914-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 17914-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17914-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 17914-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 17914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:300.

• 

  IP experiment of HeLa using 17914-1-AP

  IP result of anti-MDMX (IP:17914-1-AP, 4ug; Detection:17914-1-AP 1:600) with HeLa cells lysate 1120 ug.

• 

  IHC staining of human ovary tumor using 17914-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17914-1-AP (MDMX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 17914-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17914-1-AP (MDMX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules HeLa apoptosées, cellules HepG2, cellules Raji
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 27 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

17914-1-AP cible MDMX dans les applications de WB, IHC, IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MDMX Protéine recombinante Ag12319
• Nom complet: Mdm4 p53 binding protein homolog (mouse)
• Masse moléculaire calculée: 490 aa, 55 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC067299
• Symbole du gène: MDMX/MDM4
• Identification du gène (NCBI): 4194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kD, while the observed mass is 80 kD, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human,mouse	WB,IF	Brain; Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.; Authors - Hyojung Kim
human	WB	Cancer Res; FL118 Induces p53-Dependent Senescence in Colorectal Cancer Cells by Promoting Degradation of MdmX.; Authors - Xiang Ling
rat	WB	Redox Biol; Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.; Authors - Yuehong Wang
human	WB	Cell Death Discov; LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX; Authors - Qingqing Zhao
human	WB	J Med Chem; Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.; Authors - Shiyan Zhang
mouse	WB	PLoS Genet; MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.; Authors - Meenalakshmi Chinnam ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Breanna (Verified Customer) (02-18-2020)

It works well

• Applications: ChIP,

FH

Breanna (Verified Customer) (01-29-2020)

Works well

• Applications: Immunoprecipitation, Flow Cytometry,

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