Anticorps Polyclonal de lapin anti-MDH1

• Phare
• Validé par KD/KO

MDH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 15904-1-AP

Synonymes

MDHA, KAR, EC:1.1.1.96, EC:1.1.1.37, Aromatic alpha-keto acid reductase

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Galerie de données de validation

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  WB analysis of HepG2 using 15904-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15904-1-AP

  WB result of MDH1 antibody (15904-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MDH1 transfected HEK-293 cells.

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  WB analysis of HL-60 using 15904-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15904-1-AP (MDH1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15904-1-AP

  IP result of anti-MDH1 (IP:15904-1-AP, 4ug; Detection:15904-1-AP 1:10000) with HepG2 cells lysate 960 ug.

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  IHC staining of human renal cell carcinoma using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15904-1-AP (MDH1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15904-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15904-1-AP (MDH1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15904-1-AP

  Immunofluorescent analysis of HepG2 cells, using MDH1 antibody 15904-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 15904-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MDH1 (15904-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HL-60, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu cardiaque de rat, tissu de cancer du foie humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Informations sur le produit

15904-1-AP cible MDH1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MDH1 Protéine recombinante Ag8744
• Nom complet: malate dehydrogenase 1, NAD (soluble)
• Masse moléculaire calculée: 334 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC001484
• Symbole du gène: MDH1
• Identification du gène (NCBI): 4190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MDH1 (Malate dehydrogenase, cytoplasmic) is also named as MDHA and belongs to the LDH/MDH superfamily and MDH type 2 family which catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. It can exsit as a dimer and the dimeric MDH1 is the mitochondrial isoenzyme, whereas the tetrameric MDH2 is the glycosomal isoenzyme.(PMID:10693743)

Publications

Species	Application	Title
human	WB	Cell; An Essential Role of the Mitochondrial Electron Transport Chain in Cell Proliferation Is to Enable Aspartate Synthesis.; Authors - Kıvanç Birsoy ; KO Validated
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
mouse	WB	Blood; MDH1-mediated malate-aspartate NADH shuttle maintains the activity levels of fetal liver hematopoietic stem cells.; Authors - Hao Gu
human	WB	J Exp Med; Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.; Authors - Miao Liu
human	WB	Cell Res; cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.; Authors - Linchong Sun
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C