Anticorps Recombinant de lapin anti-MCT4

MCT4 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242296E3

N° de cat : 84797-4-PBS

Synonymes

MCT-4, SLC16A3, 242296E3, MCT 4, MCT3

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Galerie de données de validation

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  WB analysis using 84797-4-RR (same clone as 84797-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84797-4-RR (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84797-4-RR (MCT4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84797-4-RR (MCT4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 84797-4-RR (same clone as 84797-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84797-4-RR (same clone as 84797-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84797-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84797-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84797-4-RR against Human MCT4 were performed. The affinity constant is 3.02 nM.

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Informations sur le produit

84797-4-PBS cible MCT4 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MCT4 Protéine recombinante Ag18788
• Nom complet: solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
• Masse moléculaire calculée: 465 aa, 49 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC112269
• Symbole du gène: MCT4
• Identification du gène (NCBI): 9123
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

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