Anticorps Polyclonal de lapin anti-MCRS1

MCRS1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 11362-1-AP

Synonymes

58 kDa microspherule protein, ICP22BP, INO80 complex subunit J, INO80Q, MCRS1, MCRS2, microspherule protein 1, MSP58, P78

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Galerie de données de validation

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  WB analysis of Jurkat using 11362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 11362-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11362-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11362-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11362-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11362-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 11362-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11362-1-AP (MCRS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, tissu cérébral humain, tissu hépatique humain, tissu splénique de rat, tissu splénique de souris, tissu splénique humain, tissu utérin de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11362-1-AP cible MCRS1 dans les applications de WB, IHC, IF, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCRS1 Protéine recombinante Ag1904
• Nom complet: microspherule protein 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC011794
• Symbole du gène: MCRS1
• Identification du gène (NCBI): 10445
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCRS1, also named INO80Q, MSP58 or p78, is a nucleolar protein expressed in the very early S phase. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 is also present on polyribosomes of synaptoneurosome as a novel RNA-binding protein to activate the rRNA transcription. The forkhead-associated domain of MCRS1 is involved in association with centrosomal protein Nde1, comprising a component of the centrosome and taking an essential role in viability. Its isoform MCRS2 might be a linker between telomere maintenance and cell-cycle regulation by interacting with LPTS/PinX1, a telomerase-inhibitory protein. MCRS1 is detected by Western blot as a highly modified protein at 78 kDa and minor bands at about 62 and 55 kDa.

Publications

Species	Application	Title
human	WB	Cell; MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.; Authors - Aindrila Chatterjee
mouse	WB	Elife; MOF-associated complexes ensure stem cell identity and Xist repression.; Authors - Tomasz Chelmicki
mouse	WB,IF,CoIP	EMBO Rep; Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis; Authors - Jia-Qian Ju ; KO Validated
human	IF	Sci Rep; MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.; Authors - Si-Hyung Lee
human	WB	J Biol Chem; Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling.; Authors - Chen Lu L
human	WB	Mol Biol Cell; Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.; Authors - Hongdan Yang