Anticorps Polyclonal de lapin anti-MCM3

• Phare
• Validé par KD/KO

MCM3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 15597-1-AP

Synonymes

HCC5, MCM3, P1 MCM3, P1.h, p102, RLF subunit beta, RLFB

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Galerie de données de validation

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  WB analysis of K-562 using 15597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15597-1-AP (MCM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15597-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15597-1-AP (MCM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15597-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15597-1-AP (MCM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15597-1-AP

  IP result of anti-MCM3 (IP:15597-1-AP, 4ug; Detection:15597-1-AP 1:500) with HeLa cells lysate 1400ug.

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  IHC staining of human colon cancer using 15597-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15597-1-AP (MCM3 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15597-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15597-1-AP (MCM3 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules Raji
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 5 publications below

Informations sur le produit

15597-1-AP cible MCM3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MCM3 Protéine recombinante Ag7966
• Nom complet: minichromosome maintenance complex component 3
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 102-105 kDa
• Numéro d’acquisition GenBank: BC001626
• Symbole du gène: MCM3
• Identification du gène (NCBI): 4172
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). MCM3 has 2 isoforms with the molecular mass of 91 and 96 kDa. The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

Publications

Species	Application	Title
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
mouse,human	WB	Aging Cell; Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation; Authors - Jie Li ; KD Validated
human	WB	Elife; MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.; Authors - Yuichiro Saito
human	WB	Oncotarget; Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis.; Authors - Yinan Ma
human	WB	Ecotoxicol Environ Saf; The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis.; Authors - Zemin Li