Anticorps Polyclonal de lapin anti-MBP-Tag

MBP-Tag Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante et plus (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 15089-1-AP

Synonymes

Maltose Binding Protein, MBP, MBP Tag, MBP-Tag

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• Galerie de données de validation
• Proteintech vs. Other Vendors

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  WB analysis of Recombinant protein using 15089-1-AP

  lane1: MBP-NOL6 64kd; lane2 MBP 42kd were subjected to SDS PAGE followed by western blot with 15089-1-AP (MBP-Tag Antibody) at dilution of 1:3000.

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Applications testées

• Résultats positifs en WB: piste1 : MBP-NOL6 64 kd ; piste2 MBP 42 kd, Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

15089-1-AP cible MBP-Tag dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MBP-Tag Protéine recombinante Ag0942
• Nom complet: MBP-Tag
• Masse moléculaire calculée: 40 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein (MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Publications

Species	Application	Title
		Nature; Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.; Authors - Zhaoyang Li
	ELISA	Blood; Gamma-glutamyl carboxylase mutations differentially affect the biological function of vitamin K-dependent proteins.; Authors - Zhenyu Hao
	WB	Blood; γ-Glutamyl carboxylase mutations differentially affect the biological function of vitamin K-dependent proteins.; Authors - Zhenyu Hao
	WB	Sci Adv; The deubiquitinases UBP12 and UBP13 integrate with the E3 ubiquitin ligase XBAT35.2 to modulate VPS23A stability in ABA signaling.; Authors - Guangchao Liu
	WB	Cell Res; Modulation of microRNA processing by mismatch repair protein MutLα.; Authors - Mao Guogen G
		Proc Natl Acad Sci U S A; Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology.; Authors - Jianguo Yang