Anticorps Polyclonal de lapin anti-Matrilin 2

Matrilin 2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 24064-1-AP

Synonymes

MATN2, Matrilin 2

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 24064-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24064-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24064-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24064-1-AP (Matrilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

24064-1-AP cible Matrilin 2 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Matrilin 2 Protéine recombinante Ag21113
• Nom complet: matrilin 2
• Masse moléculaire calculée: 107 kDa
• Poids moléculaire observé: 120-150 kDa
• Numéro d’acquisition GenBank: BC010444
• Symbole du gène: Matrilin 2
• Identification du gène (NCBI): 4147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The matrilins represent a new family of oligomeric non-collagenous extracellular matrix proteins that are assumed to act as adapter molecules connecting other proteins and proteoglycans in the extracellular matrix (PMID: 17513098). Four matrilins (matrilin-1, -2, -3, and -4) have been identified. They all share a structure made up of von Willebrand factor A domains, EGF-like domains and a coiled-coil alpha-helical module (PMID: 18386166). Matrilin-1 and matrilin-3 are expressed mainly in cartilage, while matrilin-2 and matrilin-4 show broader tissue distribution. Matrilin-2 is the largest member of the family. It incorporates into loose and dense connective tissue and becomes associated with some basement membranes (PMID: 17513098).

Publications

Species	Application	Title
zebrafish	WB	Sci Adv; A basement membrane discovery pipeline uncovers network complexity, regulators, and human disease associations.; Authors - Ranjay Jayadev ; KD Validated
mouse	WB,IF	J Mol Med (Berl); Valproic acid modulates collagen architecture in the postoperative conjunctival scar.; Authors - Li-Fong Seet
human	WB	Int J Mol Sci; A Regulator Role for the ATP-Binding Cassette Subfamily C Member 6 Transporter in HepG2 Cells: Effect on the Dynamics of Cell-Cell and Cell-Matrix Interactions; Authors - Ilenia Matera
human	WB,CoIP	Funct Integr Genomics; MATN2 overexpression suppresses tumor growth in ovarian cancer via PTEN/PI3K/AKT pathway; Authors - Jingbo Liu