Anticorps Polyclonal de lapin anti-MAS1L

• Phare
• Validé par KD/KO

MAS1L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 20080-1-AP

Synonymes

Mas related G protein coupled receptor MRG, MAS L, MAS1L-Specific, MRG, Mas-related G-protein coupled receptor MRG

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Galerie de données de validation

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  WB analysis of HEK-293 using 20080-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 20080-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20080-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20080-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20080-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20080-1-AP (MAS1L-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 20080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20080-1-AP (MAS1L-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20080-1-AP (MAS1L-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20080-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAS1L antibody 20080-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules COLO 320, cellules HeLa, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

20080-1-AP cible MAS1L dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: MAS1 oncogene-like
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_052967
• Symbole du gène: MAS1L
• Identification du gène (NCBI): 116511
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAS1L, also named as MRG, belongs to the G-protein coupled receptor 1 family and Mas subfamily. The antibody is specific to MAS1L.

Publications

Species	Application	Title
mouse	WB	J Transl Med; ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway; Authors - Feng Chen
mouse	WB	Inflammation; ACE2 Expressed on Myeloid Cells Alleviates Sepsis-Induced Acute Liver Injury via the Ang-(1-7)-Mas Receptor Axis; Authors - Lei Liu
mouse	WB	J Inflamm Res; The ACE2-Ang-(1‑7)-Mas Axis Modulates M1/M2 Macrophage Polarization to Relieve CLP-Induced Inflammation via TLR4-Mediated NF-кb and MAPK Pathways.; Authors - Hang Pan
mouse	IHC,WB	ACS Omega; Azelaic Acid Regulates the Renin-Angiotensin System and Improves Colitis Based on Network Pharmacology and Experimentation; Authors - Yangjie Liao
rat	WB	Neural Plast; Electroacupuncture Improved Chronic Cerebral Hypoperfusion-Induced Anxiety-Like Behavior and Memory Impairments in Spontaneously Hypertensive Rats by Downregulating the ACE/Ang II/AT1R Axis and Upregulating the ACE2/Ang-(1-7)/MasR Axis.; Authors - Peipei Feng
rat	WB,IHC	Pharmacology; Canagliflozin Ameliorates Ventricular Remodeling through Apelin/Angiotensin-Converting Enzyme 2 Signaling in Heart Failure with Preserved Ejection Fraction Rats; Authors - Tingting Zhang