Anticorps Polyclonal de lapin anti-MARVELD2

MARVELD2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 13515-1-AP

Synonymes

DFNB49, MARVD2, MARVEL domain containing 2, MARVELD2, MRVLDC2, Tric, Tricellulin

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Galerie de données de validation

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  WB analysis using 13515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13515-1-AP (MARVELD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13515-1-AP

  Hela cell lysates were subjected to SDS PAGE followed by western blot with 13515-1-AP (MARVELD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 13515-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13515-1-AP (MARVELD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

13515-1-AP cible MARVELD2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MARVELD2 Protéine recombinante Ag4446
• Nom complet: MARVEL domain containing 2
• Masse moléculaire calculée: 558 aa, 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC033689
• Symbole du gène: MARVELD2
• Identification du gène (NCBI): 153562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARVELD2, also named as Tricellulin or TRIC, is a 558 amino acid protein, which contains one MARVEL domain. MARVELD2 localizes the cell membrane and plays a role in the formation of the epithelial barriers. MARVELD2, is a first integral membrane protein that is concentrated at the vertically oriented tight junction strands of tricellular contacts. MARVELD2 may be a component to maintain the integrity for peripheral nervous system myelin function and morphology.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Tricellulin deficiency affects tight junction architecture and cochlear hair cells.; Authors - Nayak Gowri G
pig	WB,IHC	Microb Pathog; Alterations in the expression level of visfatin in the lungs of piglets infected with PRRSV and its effect on PRRSV replication.; Authors - Zhe-Wei Zhang
	IF	PLoS One; Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon; Authors - David F. Russell
	IF	Tissue Cell; Papilla cells may guard the entrance to ampullary organs of Polyodon electroreceptors; Authors - David F Russell
human		Cell Rep; Alpha-tocopherylquinone differentially modulates claudins to enhance intestinal epithelial tight junction barrier via AhR and Nrf2 pathways; Authors - Ashwinkumar Subramenium Ganapathy

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Beth (Verified Customer) (10-05-2017)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RWPE1, PC-3, primary prostate epithelial cells

MARVELD2 Antibody Western Blot validation (1:1000 dilution) in RWPE1, PC-3, primary prostate epithelial cells (Cat no:13515-1-AP)