Anticorps Polyclonal de lapin anti-MARK4

• Phare
• Validé par KD/KO

MARK4 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 20174-1-AP

Synonymes

KIAA1860, MARK4, MARKL1, Nbla00650

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Galerie de données de validation

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  IHC staining of human kidney using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20174-1-AP (MARK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20174-1-AP (MARK4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:50.

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  IHC staining of human colon using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:50.

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  IHC staining of human gliomas using 20174-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20174-1-AP (MARK4 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en IHC: tissu rénal humain, tissu de côlon humain, tissu de gliome humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

20174-1-AP cible MARK4 dans les applications de IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MARK4 Protéine recombinante Ag14013
• Nom complet: MAP/microtubule affinity-regulating kinase 4
• Masse moléculaire calculée: 752 aa, 83 kDa
• Numéro d’acquisition GenBank: BC009049
• Symbole du gène: MARK4
• Identification du gène (NCBI): 57787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARK4(MAP/microtubule affinity-regulating kinase 4) is also named as KIAA1860, MARKL1 and belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. It is directly involved in microtubule organization in neuronal cells and may contribute to the pathological phosphorylation of tau in Alzheimer's disease and it also plays a role in hepatocellular carcinogenesis. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 83 kDa and 75 kDa. MARK4 can exsit as 40-65 kDa forms in hippocampal homogenates from Alzheimer`s disease and non-demented elderly cases besides the full-length isoform 84 kDa. (PMID:24533944).

Publications

Species	Application	Title
rat		FASEB J; Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization.; Authors - Wenhui Su
mouse	IF	FASEB J; NC1-peptide regulates spermatogenesis through changes in cytoskeletal organization mediated by EB1.; Authors - Shiwen Liu
rat		FASEB J; Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.; Authors - Ying Gao
rat	IF	Endocrinology; Sperm Release at Spermiation Is Regulated by Changes in the Organization of Actin- and Microtubule-Based Cytoskeletons at the Apical Ectoplasmic Specialization-A Study Using the Adjudin Model.; Authors - Linxi Li
rat		Toxicol Sci; Regulation of BTB dynamics in spermatogenesis - insights from the adjudin toxicant model.; Authors - Bai-Ping Mao
human,mouse	IHC	J Oral Microbiol; Microtubule affinity regulating kinase 4 promoted activation of the NLRP3 inflammasome-mediated pyroptosis in periodontitis.; Authors - Lulu Wang