Anticorps Polyclonal de lapin anti-MARCKSL1

MARCKSL1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 11422-1-AP

Synonymes

F52, Mac MARCKS, MACMARCKS, MARCKS like 1, MARCKS like protein 1, MARCKS related protein, MARCKSL1, MLP, MLP1, MRP

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Galerie de données de validation

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  WB analysis of human brain using 11422-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11422-1-AP (MARCKSL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11422-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11422-1-AP (MARCKSL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11422-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11422-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11422-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11422-1-AP (MARCKSL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

11422-1-AP cible MARCKSL1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MARCKSL1 Protéine recombinante Ag1967
• Nom complet: MARCKS-like 1
• Masse moléculaire calculée: 195 aa, 20 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC007904
• Symbole du gène: MARCKSL1
• Identification du gène (NCBI): 65108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, it is also named MARCKS-like protein (MLP) or MARCKS-related protein (MRP) and belongs to the MARCKS family, which is a highly acidic myristoylated family of PKC substrates widely distributed in diverse cell types including macrophages. The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation. Genetic disruption of MARCKSL1 results in neural tube closure defects, events that depend on coordinated control of actin functions, cell shape, and cell migration. MARCKSL1 are thought to regulate the actin cytoskeleton and thereby participate in major cellular responses such as phagocytosis, secretion, motility, mitogenesis, and membrane trafficking.

Publications

Species	Application	Title
mouse	WB	J Proteome Res; Sparc-like protein 1 is a new marker of human glioma progression.; Authors - Turtoi Andrei A