Anticorps Polyclonal de lapin anti-EB1

• Phare
• Validé par KD/KO

EB1 Polyclonal Antibody for WB, IP, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA, FC (Intra)

Conjugaison

Non conjugué

Publications

5

N° de cat : 17717-1-AP

Synonymes

EB1, MAPRE1, Microtubule-associated protein RP/EB family member 1, End binding protein 1, End-binding protein 1, APC binding protein EB1, APC-binding protein EB1

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Galerie de données de validation

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  WB analysis of HeLa using 17717-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17717-1-AP

  WB result of EB1 antibody (17717-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EB1 transfected HeLa cells.

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  WB analysis of Jurkat using 17717-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17717-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17717-1-AP (MAPRE1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IP experiment of Caco-2 using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with Caco-2 cells lysate 1920 ug.

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  IP experiment of mouse thymus using 17717-1-AP

  IP result of anti-EB1 (IP:17717-1-AP, 4ug; Detection:17717-1-AP 1:1000) with mouse thymus tissue lysate 2800ug.

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  IHC staining of human kidney using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17717-1-AP (MAPRE1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 17717-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17717-1-AP (EB1 Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HeLa using 17717-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EB1 (17717-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules Jurkat
• Résultats positifs en IP: cellules HeLa, cellules Caco-2, tissu de thymus de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cutané humain, tissu de cancer du côlon humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 4 publications below

Informations sur le produit

17717-1-AP cible EB1 dans les applications de WB, IP, IF, IHC, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EB1 Protéine recombinante Ag12092
• Nom complet: microtubule-associated protein, RP/EB family, member 1
• Masse moléculaire calculée: 268 aa, 30 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC106068
• Symbole du gène: EB1
• Identification du gène (NCBI): 22919
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human		Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
human	IF	Elife; α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.; Authors - Dandan Ma
mouse	IF	Biol Open; MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1; Authors - Peihong Su
mouse	IF	Biol Open; MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1.; Authors - Peihong Su
human	IF	Molecules; Pyrrolyldihydropyrazino[1,2-a]indoletrione Analogue Microtubule Inhibitor Induces Cell-Cycle Arrest and Apoptosis in Colorectal Cancer Cells; Authors - Dong-Lin Yang