Anticorps Polyclonal de lapin anti-MAPKAPK5

• Phare
• Validé par KD/KO

MAPKAPK5 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 14339-1-AP

Synonymes

MAPKAP kinase 5, MAPKAPK 5, MAPKAPK5, PRAK

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Galerie de données de validation

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  WB analysis of HeLa using 14339-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14339-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14339-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14339-1-AP cible MAPKAPK5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAPKAPK5 Protéine recombinante Ag5650
• Nom complet: mitogen-activated protein kinase-activated protein kinase 5
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC047284
• Symbole du gène: MAPKAPK5
• Identification du gène (NCBI): 8550
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAPKAPK5(MAP kinase-activated protein kinase 5) is also named as PRAK and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK5 contains the conserved protein kinase domains I through XI, which are characteristic of all protein kinases. It is a major stress-activated kinase that can phosphorylate small heat shock protein, suggesting a potential role for PRAK in mediating stress-induced HSP27 phosphorylation in vivo(PMID:9628874). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; The essential role of PRAK in tumor metastasis and its therapeutic potential.; Authors - Yuqing Wang ; KO Validated
human	WB, IP	J Cancer Biol Res; Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility.; Authors - Sheila Figel Dwyer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

hala (Verified Customer) (06-16-2022)

it works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2