Anticorps Polyclonal de lapin anti-MAP4K1

MAP4K1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 23950-1-AP

Synonymes

HPK1, MAP4K1, MEK kinase kinase 1, MEKKK 1

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Galerie de données de validation

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  WB analysis of Raji using 23950-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23950-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23950-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 23950-1-AP

  IP result of anti-MAP4K1 (IP:23950-1-AP, 4ug; Detection:23950-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 23950-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 23950-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 23950-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 23950-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

23950-1-AP cible MAP4K1 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAP4K1 Protéine recombinante Ag20221
• Nom complet: mitogen-activated protein kinase kinase kinase kinase 1
• Masse moléculaire calculée: 833 aa, 91 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC152994
• Symbole du gène: MAP4K1
• Identification du gène (NCBI): 11184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP4K1(Mitogen-activated protein kinase kinase kinase kinase 1) is also named as MEKKK 1, HPK1 and belongs to the protein kinase superfamily. It may function as a novel tumor suppressor and its loss plays a critical role in pancreatic cancer. MAP4K1 regulates stress responses, proliferation, and apoptosis in hematopoietic cells. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Signal; SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells.; Authors - Xunping Zhao
human	WB	Clin Exp Med; An HPK1 inhibitor enhanced the tumour response to anti-PD-1 immunotherapy in non-Hodgkin's lymphoma; Authors - Lin Yang
human	WB	Nat Commun; DNA framework-engineered chimeras platform enables selectively targeted protein degradation; Authors - Li Zhou