Anticorps Polyclonal de lapin anti-MEK3

• Phare
• Validé par KD/KO

MEK3 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 13898-1-AP

Synonymes

MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MAPKK3, MEK 3, MEK3, MKK3, PRKMK3

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Galerie de données de validation

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  WB analysis of HeLa using 13898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13898-1-AP (MEK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13898-1-AP (MEK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13898-1-AP

  IP Result of anti-MEK3 (IP:13898-1-AP, 4ug; Detection:13898-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IP experiment of HeLa using 13898-1-AP

  IP Result of anti-MEK3 (IP:13898-1-AP, 4ug; Detection:13898-1-AP 1:1000) with HeLa cells lysate 3200ug.

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  IHC staining of rat skeletal muscle using 13898-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13898-1-AP (MEK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skeletal muscle using 13898-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13898-1-AP (MEK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 13898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13898-1-AP (MEK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 13898-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13898-1-AP (MEK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below

Informations sur le produit

13898-1-AP cible MEK3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MEK3 Protéine recombinante Ag4861
• Nom complet: mitogen-activated protein kinase kinase 3
• Masse moléculaire calculée: 347 aa, 39 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC032478
• Symbole du gène: MAP2K3
• Identification du gène (NCBI): 5606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Identification of MAP Kinase Kinase 3 as a protein target of myricetin in non-small cell lung cancer cells; Authors - Ling Gu ; KD Validated
human	WB	Cell Mol Life Sci; HERC2 deficiency activates C-RAF/MKK3/p38 signalling pathway altering the cellular response to oxidative stress; Authors - Joan Sala-Gaston
mouse	WB	Food Funct; L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.; Authors - Kehong Liu
human	WB	Sci Rep; The ubiquitin ligase HERC1 regulates cell migration via RAF-dependent regulation of MKK3/p38 signaling.; Authors - Leonardo Pedrazza ; KD Validated
mouse	WB	Mol Immunol; Spiroplasma eriocheiris induces mouse 3T6-Swiss albino cell apoptosis that associated with the infection mechanism.; Authors - Libo Hou
human	WB	PLoS One; Proteomic Analysis of eIF5B Silencing-Modulated Proteostasis.; Authors - Xu Jiang