Anticorps Polyclonal de lapin anti-MAGEB18

MAGEB18 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 17494-1-AP

Synonymes

MAGE B18 antigen, MAGEB18, melanoma antigen family B, 18

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Galerie de données de validation

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  WB analysis using 17494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17494-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 17494-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17494-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17494-1-AP (MAGEB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17494-1-AP (MAGEB18 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 17494-1-AP

  Immunofluorescent analysis of A549 cells using 17494-1-AP (MAGEB18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A549, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17494-1-AP cible MAGEB18 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MAGEB18 Protéine recombinante Ag11624
• Nom complet: melanoma antigen family B, 18
• Masse moléculaire calculée: 343 aa, 39 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC029525
• Symbole du gène: MAGEB18
• Identification du gène (NCBI): 286514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.