Anticorps Polyclonal de lapin anti-Lin28B-specific

• Phare
• Validé par KD/KO

Lin28B-specific Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (1)

N° de cat : 16178-1-AP

Synonymes

CSDD2, lin 28 homolog B (C. elegans), Lin 28B, Lin28, LIN28B, Lin28B-specific, Protein lin 28 homolog B

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Galerie de données de validation

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  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16178-1-AP

  Immunofluorescent analysis of HepG2 cells using 16178-1-AP (Lin28B-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 10 publications below
• IF: See 1 publications below

Informations sur le produit

16178-1-AP cible Lin28B-specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lin-28 homolog B (C. elegans)
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: NM_001004317
• Symbole du gène: LIN28B
• Identification du gène (NCBI): 389421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. LIN2B shares 78% sequence identity with LIN28A. This antibody is specific to LIN28B.

Publications

Species	Application	Title
human	WB	Cancer Res; Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.; Authors - Domenico Albino
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang ; KD Validated
human	IHC	Gastric Cancer; LOC101929709 promotes gastric cancer progression by aiding LIN28B to stabilize c-MYC mRNA; Authors - Tong-Peng Xu
human	WB	Mol Ther; PMID: 29456019; Authors - Xinyi Wang
rat	IHC	Sci Rep; Testicular expression of the Lin28/let-7 system: Hormonal regulation and changes during postnatal maturation and after manipulations of puberty.; Authors - S Sangiao-Alvarellos
human	IHC	Am J Cancer Res; Cellular 5-hydroxylmethylcytosine content determines tumorigenic potential and prognosis of pancreatic ductal adenocarcinoma.; Authors - Yi-Lng Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (01-24-2023)

Antibody worked well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A172