Anticorps Polyclonal de lapin anti-Lin28A-specific

• Phare
• Validé par KD/KO

Lin28A-specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (2)

N° de cat : 16177-1-AP

Synonymes

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Lin28A-specific, Protein lin 28 homolog A, ZCCHC1

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Galerie de données de validation

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  WB analysis of hESC using 16177-1-AP

  hESC cells were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16177-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16177-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16177-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 16177-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 16177-1-AP (Lin28A-specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16177-1-AP (Lin28A-specific antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SCEh, cellule NCCIT, tissu cardiaque humain, tissu cérébral humain, tissu hépatique de souris
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu cardiaque humain, tissu cérébral humain, tissu ovarien humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

16177-1-AP cible Lin28A-specific dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: lin-28 homolog (C. elegans)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: NM_024674
• Symbole du gène: LIN28
• Identification du gène (NCBI): 79727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human LIN28.This antibody recognize Lin28A specifically.

Publications

Species	Application	Title
human	WB	Cell Death Differ; LIN28A enhances regenerative capacity of human somatic tissue stem cells via metabolic and mitochondrial reprogramming.; Authors - Kelvin Pieknell
human	WB	Cancer Res; Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.; Authors - Domenico Albino
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang ; KD Validated
human	WB	EMBO Rep; The human FOXM1 homolog promotes basal progenitor cell proliferation and cortical folding in mouse.; Authors - Wenwen Wang
human	WB	Cell Death Dis; Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A.; Authors - Qin He
human	WB	J Virol; Hepatitis C virus-induced cancer stem cell-like signatures in cell culture and murine tumor xenografts.; Authors - Ali Naushad N

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Carolina (Verified Customer) (09-26-2019)

Antibody was more specific than the previous Lin28A antibody.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:20
• Cell Tissue Type: Human Testis

FH

KUNHUA (Verified Customer) (02-01-2019)

Got a very strong band at 25-37kd with 1:1000 concentration.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human erythroid progenitor