Anticorps Polyclonal de lapin anti-Lysozyme
Lysozyme Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris
Applications
WB, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 15013-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules THP-1, cellules HL-60, tissu pulmonaire de souris, tissu splénique humain |
Résultats positifs en IHC | tissu cardiaque de souris, il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochimie (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 7 publications below |
IF | See 4 publications below |
Informations sur le produit
15013-1-AP cible Lysozyme dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Lysozyme Protéine recombinante Ag6917 |
Nom complet | lysozyme (renal amyloidosis) |
Masse moléculaire calculée | 17 kDa |
Poids moléculaire observé | 15-17 kDa |
Numéro d’acquisition GenBank | BC004147 |
Symbole du gène | Lysozyme |
Identification du gène (NCBI) | 4069 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.
Protocole
Product Specific Protocols | |
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WB protocol for Lysozyme antibody 15013-1-AP | Download protocol |
IHC protocol for Lysozyme antibody 15013-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Chem Biol Engineered Glycocalyx Regulates Stem Cell Proliferation in Murine Crypt Organoids. | ||
Arch Toxicol Extending the viability of human precision-cut intestinal slice model for drug metabolism studies. | ||
FASEB J Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate. | ||
Arch Med Sci Lysozyme promotes renal fibrosis through the JAK/STAT3 signal pathway in diabetic nephropathy | ||
Int J Mol Sci Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays. | ||
Physiol Genomics The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Balawant (Verified Customer) (12-20-2022) | I have used this antibody for colon tissue and colon cell line. this antibody is working great and no background.
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FH Claudio (Verified Customer) (12-15-2021) | The antibody staining did not yield any labelling, though it was tested in paraffin sections so you might get better results with cryostat sections.
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