Anticorps Polyclonal de lapin anti-Lysozyme

Lysozyme Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 15013-1-AP

Synonymes

1,4 beta N acetylmuramidase C, lysozyme, lysozyme (renal amyloidosis), Lysozyme C, LYZ, LZM



Applications testées

Résultats positifs en WBcellules THP-1, cellules HL-60, tissu pulmonaire de souris, tissu splénique humain
Résultats positifs en IHCtissu cardiaque de souris,
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochimie (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15013-1-AP cible Lysozyme dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Lysozyme Protéine recombinante Ag6917
Nom complet lysozyme (renal amyloidosis)
Masse moléculaire calculée 17 kDa
Poids moléculaire observé15-17 kDa
Numéro d’acquisition GenBankBC004147
Symbole du gène Lysozyme
Identification du gène (NCBI) 4069
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Protocole

Product Specific Protocols
WB protocol for Lysozyme antibody 15013-1-APDownload protocol
IHC protocol for Lysozyme antibody 15013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Chem Biol

Engineered Glycocalyx Regulates Stem Cell Proliferation in Murine Crypt Organoids.

Authors - Sara H Rouhanifard
human

Arch Toxicol

Extending the viability of human precision-cut intestinal slice model for drug metabolism studies.

Authors - C Biel
mouseWB

FASEB J

Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

Authors - Brittany R Jenkins
mouseWB

Arch Med Sci

Lysozyme promotes renal fibrosis through the JAK/STAT3 signal pathway in diabetic nephropathy

Authors - Yan Ren
mouse

Int J Mol Sci

Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.

Authors - Wenyan Yang
ratIF

Physiol Genomics

The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats.

Authors - Kari Chesney

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for colon tissue and colon cell line. this antibody is working great and no background.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: colon tissue and colon cell line (caco2 cells)
FH

Claudio (Verified Customer) (12-15-2021)

The antibody staining did not yield any labelling, though it was tested in paraffin sections so you might get better results with cryostat sections.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse heart sections