Anticorps Polyclonal de lapin anti-LYPLA1

LYPLA1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16055-1-AP

Synonymes

Acyl protein thioesterase 1, APT 1, APT1, hAPT1, LPL I, LPL1, LYPLA1, Lysophospholipase 1, lysophospholipase I, LYSOPLA, LysoPLA I

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Galerie de données de validation

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  WB analysis using 16055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16055-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16055-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16055-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16055-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16055-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16055-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16055-1-AP (LYPLA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16055-1-AP

  IP result of anti-LYPLA1 (IP:16055-1-AP, 4ug; Detection:16055-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 16055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16055-1-AP (LYPLA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16055-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16055-1-AP (LYPLA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16055-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16055-1-AP (LYPLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu hépatique de rat, tissu hépatique humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below

Informations sur le produit

16055-1-AP cible LYPLA1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LYPLA1 Protéine recombinante Ag9022
• Nom complet: lysophospholipase I
• Masse moléculaire calculée: 230 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC010397
• Symbole du gène: LYPLA1
• Identification du gène (NCBI): 10434
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LYPLA1(Lysophospholipase 1) is also named as APT1(Acyl-protein thioesterase 1), LPL1 and belongs to the AB hydrolase 2 family. It is an important enzyme responsible for depalmitoylation of palmitoyl proteins and works as a thioesterase that cleaves the S-palmitoyl group of H-Ras, heterotrimeric G protein alfa subunit (Ga) , regulator of G protein signaling 4 (RGS4) and the endothelial isoform of nitric oxide synthase (eNOS) in vitro(PMID:19439193).

Publications

Species	Application	Title
human	IHC	J Transl Med; Identifying prognostic markers in spatially heterogeneous breast cancer microenvironment; Authors - Guohong Liu
human	WB	Ecotoxicol Environ Saf; PM2.5 promoted lipid accumulation in macrophage via inhibiting JAK2/STAT3 signaling pathways and aggravating the inflammatory reaction.; Authors - Liwei Yang
human	WB	PLoS One; Development of an activity-based probe for acyl-protein thioesterases.; Authors - Megan Garland
human	IHC	Drug Metab Dispos; Identification of Bioactivating Enzymes Involved in the Hydrolysis of Laninamivir Octanoate, a Long-acting Neuraminidase Inhibitor, in Human Pulmonary Tissue.; Authors - Kumiko Koyama
human	WB	Autophagy; Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1; Authors - Ying Liu
human	IHC,WB	Reprod Sci; Estrogen Regulates Scribble Localization in Endometrial Epithelial Cells Through Acyl Protein Thioesterase (APT)-Mediated S-Palmitoylation in Adenomyosis; Authors - Zhixing Jin